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We use viral tools to label and trace circuits in the developing forebrain in vivo.
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We use genetic tools to alter gene expression in the neurons and glia of the embryonic forebrain in vivo, modulating synaptic adhesion protein expression
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We manipulate and measure activity in neurons using DREADDs and GCamp6
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We use immunohistochemistry to measure protein expression changes in glial development and myelination
We use electrophysiology to examine neuronal-glial synaptic connectivity
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We use primary cultures to examine the process of myelination
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We use RNAScope to study gene expression and glial cell fate
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​In Vivo
Examining the role of neuronal activity in neuronal-glial synapse formation and myelination in the murine forebrain
Determining how neuronal inputs regulate oligodendrocyte development and myelination, using synaptic adhesion proteins to perturb synaptic function
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In Vitro:
Using primary neuron- OPC co-cultures to measure OPC maturation and myelination of individual axons
In Lab
