We use viral tools to label and trace circuits in the developing forebrain in vivo.
We use genetic tools to alter gene expression in the neurons and glia of the embryonic forebrain in vivo, modulating synaptic adhesion protein expression
We manipulate and measure activity in neurons using DREADDs and GCamp6
We use immunohistochemistry to measure protein expression changes in glial development and myelination
We use electrophysiology to examine neuronal-glial synaptic connectivity
We use primary cultures to examine the process of myelination
We use RNAScope to study gene expression and glial cell fate