We use:
Viral tools to label and trace circuits in the developing forebrain in vivo.
Genetic tools to alter gene expression in the neurons and glia of the embryonic forebrain in vivo, modulating synaptic adhesion protein expression
Immunohistochemistry to measure protein expression changes in glial development and myelination
Electrophysiology to examine neuronal-glial synaptic connectivity
Primary cultures to examine the process of myelination
RNAScope to study gene expression and glial cell fate
We manipulate and measure activity in neurons using DREADDs and GCamp6
In Vivo
Examining the role of neuronal activity in neuronal-glial synapse formation and myelination in the murine forebrain
Determining how neuronal inputs regulate oligodendrocyte development and myelination, using synaptic adhesion proteins to perturb synaptic function
In Vitro:
Using primary neuron- OPC co-cultures to measure OPC maturation and myelination of individual axons
In Lab:
