We use viral tools to label and trace circuits in the developing forebrain in vivo.

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We use genetic tools to alter gene expression in the neurons and glia of the embryonic forebrain in vivo, modulating synaptic adhesion protein expression

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We manipulate and measure activity in neurons using DREADDs and GCamp6

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We use immunohistochemistry to measure protein expression changes in glial development and myelination

 

We use electrophysiology to examine neuronal-glial synaptic connectivity

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We use primary cultures to examine the process of myelination 

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We use RNAScope to study gene expression and glial cell fate

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